. 2013 Oct 24;8(3):601–612. doi: 10.1038/ismej.2013.182

Table 1. Strains and plasmids used in this study.

Strain or plasmid	Description and characteristics						Reference, Accession no.a
Plasmids	Groupb	Size (kb)	Resistance phenotypec	Transmission	Origin	Year
pNI105	NA	5	Neo^r, Kan^r	Non-transmissibled	Soil	1991	(Itoh et al., 1991) AB032347
pBS228	IncP-1α	89	Str^r, Car^r, Tet^r	Mobilizable	Waste water	1981	(Haines et al., 2007) NC_008357
Rms149	IncP-6	57	Str^r, Gen^r, Car^r	Mobilizable	Clinical	1975	(Haines et al., 2005) NC_007100
pAKD1	IncP-1β	58	Str^r, Hg^r	Conjugative	Soil	1998	(Sen et al., 2011) JN106164
PAMBL-1	ND	30	Gen^r, Car^r, Caz^r, Mer^r, Hg^r	Conjugative	Clinical	2005–2007	(Tato et al., 2010) GQ422829
PAMBL-2	ND	30	Str^r, Gen^r, Car^r, Caz^r, Mer^r	Conjugative	Clinical	2005–2007	(Tato et al., 2010) GQ422828

Strains
P. aeruginosa PAO1		Susceptible strain used as a recipient for plasmids					NC_002516
P. aeruginosa PAO1-GFP		PAO1 tagged with the green fluorescent protein (GFP) gene integrated at the Tn7 insertion site. Gm^r, Chl^r					(Choi and Schweizer, 2006)
PAO1/ pNI105		Neo^r, Kan^r					This work
PAO1/ pBS228		Str^r, Car^r, Tet^r					This work
PAO1/ Rms149		Str^r, Gen^r, Car^r					This work
PAO1/ pAKD1		Str^r, Hg^r					This work
PAO1/ PAMBL-1		Gen^r, Car^r, Caz^r, Mer^r, Hg^r					This work
PAO1/ PAMBL-2		Str^r, Gen^r, Car^r, Caz^r, Mer^r					This work
PAO1/ pNI105 pBS228		Neo^r, Kan^r, Str^r, Car^r, Tet^r					This work
PAO1/ pNI105 Rms149		Neo^r, Kan^r, Gen^r, Str^r, Car^r					This work
PAO1/ pNI105 pAKD1		Neo^r, Kan^r, Str^r, Hg^r					This work
PAO1/ pNI105 PAMBL-1		Neo^r, Kan^r, Gen^r, Car^r, Caz^r, Mer^r, Hg^r					This work
PAO1/ pNI105 PAMBL-2		Neo^r, Kan^r, Str^r, Gen^r, Car^r, Caz^r, Mer^r					This work

Abbreviations: NA, not applicable; ND, not determined.

Sequences present in GenBank for PAMBL-1, PAMBL-2 and pNI105 are partial.

Small plasmid pNI105 does not have a defined group, it codes for a rolling circle replication-like rep-protein.

Gen, gentamycin; Chl, chloramphenicol; Neo, neomycin; Kan, kanamycin; Str, streptomycin; Car, carbenicillin; Tet, tetracycline; Hg, mercury; Caz, ceftazidime; Mer, meropenem. ^r, high-level resistance phenotype.

The small plasmid pNI105 showed a conjugation frequency <10⁻⁷ transconjugants per donor colony-forming unit (no transconjugant detected in the conjugation assay) when coexisting with the conjugative plasmids pAKD1, PAMBL-1 and PAMBL-2.