Table 1. Strains and plasmids used in this study.
Strain or plasmid |
Description and characteristics |
Reference, Accession no.a | |||||
---|---|---|---|---|---|---|---|
Plasmids | Groupb | Size (kb) | Resistance phenotypec | Transmission | Origin | Year | |
pNI105 | NA | 5 | Neor, Kanr | Non-transmissibled | Soil | 1991 | (Itoh et al., 1991) AB032347 |
pBS228 | IncP-1α | 89 | Strr, Carr, Tetr | Mobilizable | Waste water | 1981 | (Haines et al., 2007) NC_008357 |
Rms149 | IncP-6 | 57 | Strr, Genr, Carr | Mobilizable | Clinical | 1975 | (Haines et al., 2005) NC_007100 |
pAKD1 | IncP-1β | 58 | Strr, Hgr | Conjugative | Soil | 1998 | (Sen et al., 2011) JN106164 |
PAMBL-1 | ND | 30 | Genr, Carr, Cazr, Merr, Hgr | Conjugative | Clinical | 2005–2007 | (Tato et al., 2010) GQ422829 |
PAMBL-2 | ND | 30 | Strr, Genr, Carr, Cazr, Merr | Conjugative | Clinical | 2005–2007 | (Tato et al., 2010) GQ422828 |
Strains | |||||||
P. aeruginosa PAO1 | Susceptible strain used as a recipient for plasmids | NC_002516 | |||||
P. aeruginosa PAO1-GFP | PAO1 tagged with the green fluorescent protein (GFP) gene integrated at the Tn7 insertion site. Gmr, Chlr | (Choi and Schweizer, 2006) | |||||
PAO1/ pNI105 | Neor, Kanr | This work | |||||
PAO1/ pBS228 | Strr, Carr, Tetr | This work | |||||
PAO1/ Rms149 | Strr, Genr, Carr | This work | |||||
PAO1/ pAKD1 | Strr, Hgr | This work | |||||
PAO1/ PAMBL-1 | Genr, Carr, Cazr, Merr, Hgr | This work | |||||
PAO1/ PAMBL-2 | Strr, Genr, Carr, Cazr, Merr | This work | |||||
PAO1/ pNI105 pBS228 | Neor, Kanr, Strr, Carr, Tetr | This work | |||||
PAO1/ pNI105 Rms149 | Neor, Kanr, Genr, Strr, Carr | This work | |||||
PAO1/ pNI105 pAKD1 | Neor, Kanr, Strr, Hgr | This work | |||||
PAO1/ pNI105 PAMBL-1 | Neor, Kanr, Genr, Carr, Cazr, Merr, Hgr | This work | |||||
PAO1/ pNI105 PAMBL-2 | Neor, Kanr, Strr, Genr, Carr, Cazr, Merr | This work |
Abbreviations: NA, not applicable; ND, not determined.
Sequences present in GenBank for PAMBL-1, PAMBL-2 and pNI105 are partial.
Small plasmid pNI105 does not have a defined group, it codes for a rolling circle replication-like rep-protein.
Gen, gentamycin; Chl, chloramphenicol; Neo, neomycin; Kan, kanamycin; Str, streptomycin; Car, carbenicillin; Tet, tetracycline; Hg, mercury; Caz, ceftazidime; Mer, meropenem. r, high-level resistance phenotype.
The small plasmid pNI105 showed a conjugation frequency <10−7 transconjugants per donor colony-forming unit (no transconjugant detected in the conjugation assay) when coexisting with the conjugative plasmids pAKD1, PAMBL-1 and PAMBL-2.