Figure 2.

Reduced positive selection of regulatory T cells in mice bearing the Zap70^A243V variant. Wild-type, Zap70^rps, Zap70^rps/mrt and Zap70^mrt mice were assessed for thymic function at 5–13 weeks of age for T-cell subpopulations (n = 14–21, 18–25, 7–11, 3–7 mice/group). (a) Representative flow cytometry plots for CD4 versus CD8. (b) Percentage of CD4⁺ CD8⁺ double-positive (DP) thymocytes in mice of each genotype (median with individual values shown). (c) Percentage of DP thymocytes expressing CD69 in mice of each genotype (median with individual values shown). (d) Percentage of CD4⁺ single-positive (SP) thymocytes in mice of each genotype (median with individual values shown). (e) Percentage of CD8⁺ SP thymocytes in mice of each genotype (median with individual values shown). (f) Percentage of Foxp3⁺ regulatory T cells within the CD4⁺ SP population in mice of each genotype, with the exception of Zap70^mrt mice where the parent population was absent (NA) (median with individual values shown). *P < 0·05; **P < 0·01; ***P < 0·001; ****P < 0·0001.