Figure 2.

Converted CD4⁺ H-Y TCR⁺ cells are not activated by male antigen. (a) Sorted CD8^lo H-Y TCR⁺ cells and CD4⁺ H-Y TCR⁺ cells from H-Y TCR and H-Y ThPOK transgenic mice were labelled with CFSE and cultured with mitomycin C treated spleen cells from B6 female mice with the indicated concentration of H-Y peptide. Cells were analysed by FACS at 72 and 90 hr. (b) Sorted CD8^lo H-Y TCR⁺ cells and CD4⁺ H-Y TCR⁺ cells from H-Y TCR and H-Y ThPOK transgenic mice were labelled with CFSE and activated with concanavalin A (2 μg/ml) for 48 and 60 hr. Results are representative of three independent experiments.