Figure 3.

ES-62 modulates the recruitment of B cells to the joints of mice with collagen-induced arthritis (CIA). Cells extracted from the joints of mice with CIA were analysed for the proportion (a,b) and number (c) of infiltrating CD19⁺ B cells (data in c are presented as the means ± SEM of four biological replicates pooled from two independent experiments) and consequently for the relative proportions of CD19⁺ CD23⁺ (d,e) and plasma cells on the basis of CD19 CD138 expression (f,g). Exclusion of myeloid and T-cell-expressing CD138 cells by use of Dump channel (CD4⁺ CD8⁺ GR1⁺ F4/80⁺ CD11b⁺ CD11c⁺; h) allowed analysis of Dump⁻ CD19⁻ B220⁻ CD138⁺ and Dump⁻ CD19⁺ B220^low/− CD138⁺ plasma cells (i,j).