Figure 4.

Antagonistic effects of compounds A/G against BDNF in SH-Sy5Y/TrkB cells. (A) Growth inhibitory effect mediated by the compounds A and G in the presence or absence of BDNF. The data plotted relative to the cell number without compound (set to 1). Colony formation assay (B) and cell invasion assay (C) indicating an antagonistic effect between the compounds and BDNF. In the cell invasion assay, the data plotted relative to the value at day 0 (set to 1). The P-values between the diamond (0 μmol/L) and cross mark (1 μmol/L) at day 5 were less than 0.05 in all experiments except compound A without BDNF (top left panel). (D) The candidate compounds inhibit phospho-activation of TrkB, which is mediated by BDNF. Cells were pretreated with or without compuond G (100 μmol/L) for 5 min, and were incubated with various concentrations of BDNF (0, 5, 15, 24 ng/mL) for 10 min.