Fig 2.

Effects of epidermal growth factor (EGF) on the production of CXCL5 and CXCL8 in hepatocellular carcinomas (HCCs). Protein levels of CXCL5 (A) and CXCL8 (B) in the supernatant harvested from HCC challenged with PBS (Controls) or EGF at 10, 50 or 100 ng/ml for 24 hrs (A1 and B1), 6, 12 and 24 hrs after EGF stimulation at 100 ng/ml (A2 and B2), or during each 6-hr period (e.g. 0–6, 6–12, 12–18 and 18–24 hrs) after EGF stimulation at 100 ng/ml (A3 and B3). During every 6-hour period, the full supernatant was harvested to evaluate every 6-hour production of CXCL5 and CXCL8 and the same volume of fresh solution was added.