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Monodimensional zymography (1-DZ) on 7.5% polyacrylamide containing 0.1% gelatin. Lane HC: 2 μl of serum from healthy control; Lane 1: 2 μl of serum from a patient with inactive RR-MS; Lane 2: 10 ng of recombinant human proMMP-9 produced in insect cells; Lane 3: 10 ng human recombinant pro-MMP-9 produced in CHO cells.
