Fig 6.

2-DZ analysis of gelatinase forms before and after concanavalin-A-Sepharose chromatography. 75 μl of a 10 ×  concentrated pool of sera from two patients with active RR-MS were subjected to concanavalin-A-Sepharose chromatography as described in the methods. Fractions obtained were desalted and concentrated and then analysed by 2-D zymography. 25 μl of samples were loaded on the gels. (A) concentrated pool before concanavalin-A-Sepharose chromatography; (B) unbound fraction (‘pass through’) corresponding to MMP-2; (C) wash step, with residual MMP-2; (D) eluted fraction from concanavalin-A-Sepharose chromatography.