Table 1.
2-DZ analysis of MMP-2 and effect of treatment with phosphatase
| pI |
|||
|---|---|---|---|
| Spot | Relative zymolytic level | Without phosphatase | After phosphatase treatment |
| 1 | 18 | 5.005 | 5.129 |
| 2 | 13 | 5.048 | 5.175 |
| 3 | 52 | 5.110 | 5.228 |
| 4 | 13 | 5.169 | 5.276 |
| 5 | 5 | 5.226 | 5.330 |
RR-MS pooled sera, enriched in gelatinases by affinity chromatography on gelatine-Sepharose, were incubated (O/N, at 37°C) with alkaline phosphatase (Sigma-Aldrich), and then analysed by 2-DZ. The isoelectric point of the digested spots was measured by the ImageMaster2D Elite V. 2002.01 software (Amersham Biosciences).