Table 2.
Total pattern of different forms of gelatinases detected in MS sera
| Gelatinase isoforms | Charge variants | pI | Mw | |
|---|---|---|---|---|
| Gelatinase B (MMP-9) | Multimeric form | Several (unresolved) | 4.2–4.5 | >200 |
| NGAL-pro-MMP-9 complex | 1 | 5.61 | 116 | |
| 2 | 5.72 | 116 | ||
| 3 | 5.80 | 116 | ||
| 4 | 5.92 | 116 | ||
| pro-MMP-9 | Several (unresolved) | 4.1–4.6 | 90–92 | |
| 82 kD MMP-9 | Several (unresolved) | 4.1–4.6 | 80–82 | |
| 65 kD MMP-9 | 1 | 4.82 | 65 | |
| 2 | 4.86 | 65 | ||
| 3 | 4.92 | 65 | ||
| 4 | 4.99 | 65 | ||
| 5 | 5.06 | 65 | ||
| 6 | 5.15 | 65 | ||
| Gelatinase A (MMP-2) | MMP-2 | 1 | 4.97 | 68 |
| 2 | 5.03 | 68 | ||
| 3 | 5.09 | 68 | ||
| 4 | 5.16 | 68 | ||
| 5 | 5.21 | 68 |
Image analysis of 2-DZ was carried out by the ImageMaster2D Elite V. 2002.01 software (Amersham Biosciences).