Fig 4.

Dental pulp stromal cells and periodontal ligament stromal cells do not evoke a strong immune reaction in one-way mixed lymphocyte reactions. Mitomycin-fixed stromal cells (SC) either primed or unprimed with IFNγ (150 U/ml) or TNFα ng/ml were co-cultured with allogeneic PBMCs at different dose ratios for 6 days and, subsequently, BrdU incorporation was quantified by fluorimetry. Proliferation responses to mitomycinC-treated allogeneic PBMCs were considered 100%. Data are representative of at least two independent experiments.