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. 2013 Aug 1;140(15):3210–3220. doi: 10.1242/dev.096271

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Fluorescence labeling of Tribolium embryos with photoconvertible ABP-tdEosFP. (A-A′) Optical sections through the blastoderm of a transgenic embryo ubiquitously expressing nuclear GFP (Sarrazin et al., 2012), which was injected with ABP-tdEosFP mRNA and photoconverted in the central region. (A) nGFP and unconverted ABP-tdEosFP fluorescence detected at similar levels in the green channel, (A′) photoconverted ABP-tdEosFP fluorescence detected in the red channel and (A′) overlay of the two channels. (B-B′) Optical sections through developing limb-buds of an embryo 26 hours after injection with ABP-tdEosFP mRNA. Most of the protein in the bottom limb-bud has been photoconverted. (B) Green channel showing unconverted ABP-tdEosFP, (B′) red channel showing converted ABP-tdEosFP, and (B′) overlay of the two channels. (C-C′) Optical sections through the same limbs shown in B-B′ 22 hours later. Both green unconverted and red converted ABP-tdEosFP are detected in the bottom photoconverted limb. Anterior is towards the top in all panels, and ventral midline is towards the left in B-C′. Scale bars: 25 μm.