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. 2014 Feb 20;10(2):e1004175. doi: 10.1371/journal.pgen.1004175

Figure 6. sup-9(n1435) is mutated in a conserved SC box at the C-terminus of two-pore domain K+ channels.

Figure 6

(A) Top, schematic diagram of the proposed structure of the SUP-9 channel. Cylinders represent the four presumptive transmembrane domains. Middle, alignment of the C-terminal cytoplasmic tail of SUP-9 (amino acids 247–329) and related channels: human TASK-1 (aa 248–394) Genebank Acc#NP_002237; human TASK-3 (aa 248–374) Genebank Acc#Q9NPC2; Drosophila predicted protein CG9637 (aa 238–398) Genebank Acc# AAF54970; Drosophila predicted protein CG9361 (aa 249–340) Genebank Acc# AAF54374; C. elegans TWK-4 (aa 255–334) Genebank Acc#AAC32857. Residues conserved among at least four of the channels are shaded. Box, SC box; black triangle, site of the n1435 mutation; white triangle, site of SUP-9::TWK-4 C-terminal fusion. Bottom, alignment of SC boxes from SUP-9 and related channels. Residues conserved among SUP-9 and at least two other channels are shaded. (B, C) Mutant animals of the indicated genotypes were assayed for egg-laying rate. Young adult hermaphrodites were allowed to lay eggs for 3 hrs on a bacterial lawn, and eggs or larvae on the plate were counted. Independently derived transgenic lines of (B) sup-10(n983gf) lin-15 or (C) unc-93(e1500gf) sup-18(n1030); lin-15 animals containing both a lin-15-rescuing transgene and the Pmyo-3 sup-9 derivatives are indicated. Error bars, means ± SEMs of at least 14 animals.