Table 3. sup-18 loss-of-function mutations.
Allele | Mutation | Effect | Mutagen | Background |
n1033 | ATG to ATT | M1I | EMS | sup-10(n983gf) |
n1030 | CGA to TGA | R65stop | EMS | sup-10(n983gf) |
n1038 | 916 bp deletion | 97+frameshift | EMS | unc-93(e1500gf) |
n527 | 13 bp deletion | 154+frameshift | Spont | unc-93(e1500gf) |
n1548 | TGG to TAG | W170stop | EMS | sup-10(n983gf) |
n463 | 4 bp deletion | 175+frameshift | Spont | unc-93(e1500gf) |
n1539 | Tc3 Insertion | 320+frameshift | Spont | sup-10(n983gf) |
n1036 | agGT to aaGT | 3rd splice acceptor | EMS | sup-10(n983gf) |
n1035 | agGC to aaGC | 5th splice acceptor | EMS | sup-10(n983gf) |
n1015 | GTgt to GTat | 8th splice donor | EMS | sup-10(n983gf) |
n1558 | agAT to aaAT | 8th splice acceptor | EMS | sup-10(n983gf) |
n1010 | AGT to AAT | S137N | EMS | sup-10(n983gf) |
n1554 | GGC to AGC | G258D | EMS | sup-10(n983gf) |
n1471 | GGC to GAC | G258S | Gamma | sup-10(n983gf) |
n1556 | ACT to ATT | T271I | EMS | sup-10(n983gf) |
n1014 | GGA to AGA | G280R | EMS | sup-10(n983gf) |
n1022 | AGG to AAG | R289K | EMS | sup-10(n983gf) |
n528 | ACC to CCC | T322P | Spont | unc-93(e1500gf) |
DNA sequences were determined for both strands of sup-18 exons and intron/exon boundaries of each mutant. For splice-junction mutants, the intron sequence is indicated by lowercase and the exon sequence by uppercase letters. EMS, ethyl methanesulfonate; Spont, spontaneous. Frameshift, mutations causing frameshift after the indicated codons.