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. 2014 Feb 20;10(2):e1004151. doi: 10.1371/journal.pgen.1004151

Figure 6. Transcriptional regulation of MHC class I genes.

Figure 6

(A) Allele-specific primers (Prim.) used for quantitative RT-PCR showed minimal amplification of other RT1-A alleles and were therefore considered class Ia-specific (i.e. not cross-reacting to class Ib genes); PCR products (Prod.) are shown for DA (a), DA.1UR83 (u), DA.1HR83 (h) and DA.1IR85 (i). (B) Expression in spleen of RT1-A genes in DA and Tcs1-congenic strains relative to the expression of beta-2-microglobuline (B2M). (C) Variation (fold-change) in RT1-A gene expression between different congenic strains. Data show the mean expression of 2–4 different primer sets per target gene after normalization to 3 reference genes (Table S3). Significant differences compared to RT1-Aa. (D) Expression of RT1-Aa (class Ia) and clone 3.6 (class Ib) in spleen from DA rats. The amplification of a product in DA.1HR83 but not in DA.1H (1H) indicates that the primers for clone 3.6 are not cross-reacting to the RT1-Aa gene in DA (adjacent figure).