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. 2014 Feb 20;10(2):e1004151. doi: 10.1371/journal.pgen.1004151

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© 2014 Tuncel et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Recent thymic emigrants (RTEs) express CD90 and low levels of CD45RC. Counterplots show CD8 RTEs (gated) in the spleen of 6.5-week old congenic and DA rats (representative samples, numbers in gates show percent of CD8 T cells). Scatterplot shows total number of CD8 RTEs per spleen, which correlates to the absolute number of CD8SP TCR^hi cells in the thymi from the same animals (far right). (B) The corresponding staining (as shown in A) for CD4 RTEs, including total numbers (scatterplot) and correlation to CD4SP TCR^hi cells in the thymus. (C) Absolute numbers of CD4 (blue) and CD8 (red) T cells in the spleen of 13-week old rats. Strains and genotypes are shown above and below graphs, respectively; n = 5–7 per group.