Table 1. CD4∶CD8 T cell ratio and extracellular MHC class I and II expression in spleens of congenic and recombinant strains.
| RT1a | MHC-IIb , d | |||||||||
| Strain | n | A | B | D | CEM | CD4∶CD8 | MHC-Ib , c | RT1-B | RT1-D | |
| WT | DA | 10 | a | a | a | a | 4.1±0.3 | 100±12 | 100±5.4 | 100±7.4 |
| PS | DA.1F | 10 | f | f | f | f | 2.9±0.2e | 83±7.0f | 67±4.9e | 98±6.6 |
| PS | DA.1H | 6 | h | h | h | h | 7.5±0.4f | 92±4.3 | 74±4.8e | 88±3.9f |
| PS | DA.1I | 10 | i | i | i | a | 6.9±0.4e | 67±5.4e | 97±7.2 | 97±5.6 |
| PS | DA.1U | 7 | u | u | u | u | 2.4±0.3e | 92±6.0 | 79±6.5e | 96±5.4 |
| RCS | DA.1FR9 | 7 | a | f | f | a | 2.9±0.1e | 96±8.0 | 58±4.3e | 101±16 |
| RCS | DA.1HR10 | 6 | a | h | h | a | 3.8±0.3 | 75±7.0f | 78±8.5f | 85±5.3f |
| RCS | DA.1IR7 | 9 | a | i | i | a | 4.4±0.4 | 103±4.7 | 102±13 | 100±9.4 |
| RCS | DA.1UR10 | 7 | a | u | u | a | 3.0±0.1e | 79±3.8f | 70±2.3e | 107±8.0 |
| RCS | DA.1HR83 | 5 | h | a | a | a | 7.1±0.6f | 85±6.0g | 101±11 | 98±7.1 |
| RCS | DA.1IR85 | 5 | i | a | a | a | 8.2±0.7f | 68±1.1f | 104±0.9 | 101±3.6 |
| RCS | DA.1UR83 | 5 | u | a | a | a | 3.2±0.2f | 88±2.4g | 103±4.5 | 100±4.1 |
a
RT1 haplotype designations are based on genotyping data (see Methods);
b
Strains were compared in separate experiments to DA littermate controls. The mean fluorescent intensity was then normalized to the expression of DA, which was given an arbitrary value of 100.
c
MHC-I (class Ia and Ib; OX18) extracellular expression on WBCs.
d
MHC class II expression on B cells.
Significant differences compared to DA:
e
P<0.001;
f
P<0.01;
g
P<0.05.
WT, wildtype; PS, parental strain; RCS, recombinant congenic strain. Shown are mean values ±SD.