Table 2. trans eQTL hotspots.

					Low expression sterile		High expression sterile
Chr	Position cM	Position Mb1	No. trans eQTL	“sterile” allele2	No. trans eQTL	Expression pattern3	No. trans eQTL	Expression pattern3	Sterility QTL overlapping4
2	26–38	78.68–119.28	660	D	293	F1.U; MIB; MEC; PMA,C,D	367	F1.O; SOA,D; MIA,D	TWD
3	38–44	86.05–105.42	187	M	107	F1.U; MEA; PMA	80	F1.O; SOA,B; MIA	none
5	68–71.50	143.82–148.35	187	D	118	F1.U; MEA; PMA,C,D	69	F1.O; MIA,B; MED	DBTD, TASD
6	30–34	89.94–97.3	159	D	110	F1.U; MIB; PMA,C,D	49	F1.O; MIA,D	none
10	4–24*	36.31–78.1	1,328	D	487	F1.U; MEA; PMA,D	840	F1.O; SOA,B,D; MIA,D; MED	PBTD, TWD
11	54–62	78.32–97.18	599	D	332	F1.U; SOD; MEA; PMD	267	F1.O; SOA,B; MIA,D	none
15	18–38	61.22–88.35	1,551	D	557	F1.U; MEA; PMD	994	F1.O; SOA,D; MIA,D	TASM
15	46–50	94.63–97.35	147	D	40	F1.U; SOD; MEA,C; PMB,C,D	107	F1.O; SOA; MIA,B; MEB	none
17	0–16	3.06–38.01	2,435	H	950	F1.U; MEA,C; PMA,C,D	883	F1.O; SOA; MIA,D; MED	SDH, TWH
				M	299	F1.O; F1.U; MEA	303	F1.O; F1.U; SOA; MIA,D
X	0–42	10.16–101.19	8,286	M	4239	F1.U; MEA,C; PMA,C,D	4046	F1.O; SOA,B,D; MIA,B,D; MED	PC1M, DBTM, ASHM, PBTM, H/TM, SDM
X	44–66	106.91–164.43	1,180	D	624	F1.U; PMA	556	ME6	PC1M

¹interpolated.

²allele with expression pattern consistent with sterility.

³QTT significantly enriched (hypergeometric test with Bonferroni correction) for transcripts misexpressed in MxD F1s or expressed in a spermatogenic cell type. F1.U - underexpressed in MxD F1s; F1.O – overexpressed in MxD F1s; SO – somatic cell expression; MI – mitotic cell expression; ME – meiotic cell expression; PM – postmeiotic cell expression. Superscript indicates citation for cell types: ^A [28]; ^B [17]; ^C [107]; ^D [108].

⁴single or MQM, sterile allele is indicated in superscript: D – domesticus, M – musculus, H- underdominant [1].

^*two adjacent regions from 4–16 cM and 16–24 cM combined; sliding window from 14–18 cM is not significantly enriched.