Table 3. Functional annotation of QTT associated with trans eQTL hotspots.

Chr	Position cM	Sterile allele	Low expression sterile functional Annotation*	High expression sterile functional Annotation*
2	26–36	D		glycoprotein (165); protein kinase C binding (5); phosphoprotein (165); macrophage (29); cytokine-cytokine receptor interaction (15)
3	38–44	M		glycoprotein (26)
5	68–71.50	D
6	30–34	D
10	4–24	D	cytoskeleton (45); microtubule cytoskeleton (22); G protein-coupled olfactory receptor, class II (19)	transmembrane (285)
11	54–62	D	chromatin (10)	transmembrane (95); glycoprotein (89); oxidoreductase (26); mitochondrion (25); lipid biosynthetic process (24); fatty acid metabolism (14); butanoate metabolism (14); lysosome (13); NADP (11); arginine and proline metabolism (6); lipid metabolism (5); steroidogenesis (4); intramolecular oxidoreductase activity, transposing C = C bonds (4); PPAR signaling pathway (6)
15	18–38	D	plasma membrane (63)	membrane (326); glycoprotein (256); mitochondrion (118); microsome (51); response to organic substance (44); iron (35); NAD (29); flavoprotein (27); lipid metabolism (26); gland development (25); steroid metabolic process (22); NADP-binding domain (21); gland morphogenesis (14); valine, leucine and isoleucine degradation (12); oxidation reduction (76); oxidoreductase (68); endoplasmic reticulum (59); cofactor binding (28); NADP (23); heart morphogenesis (14); binding site:substrate via amide nitrogen (8)
15	46–50	D
17	0–16	H	spermatogenesis (36); microtubule organizing center (20); alternative splicing (308); splice variant (305)	nucleus (240); ubiquitin conjugation (42); RNA-binding (39); lipid synthesis (23); NAD (21); ATP (21); lipid metabolism (18); melanosome (17); sterol metabolic process (15); phosphoprotein (460); cytoplasm (184); acetylation (177); endoplasmic reticulum (86); repressor (36); methylation (21); isomerase (18); nucleotide binding (12); P-loop (12)
		M		oxioreductase (25); fatty acid metabolism (16); steroid biosynthetic process (8); valine, leucine and isoleucine degradation (7); active site: proton acceptor (26); generation of precursor metabolites and energy (14); NAD (10)
X	0–42	M	sexual reproduction (120); secretory granule (33); fertilization (29); alternative splicing (930); splice variant (927); acrosomal vesicle (21); flagellum (20); microtubule-based flagellum (14)	membrane (1016); metal-binding (532); mitochondrion (411); protein transport (408); nucleotide-binding (373); transcription regulation (354); transferase (294); organelle lumen (274); regulation of transferase activity (168); small GTPase mediated signal transduction (161); cell fraction (144); vesicle (137); apoptosis (133); membrane fraction (116); actin-binding (99); hemopoietic or lymphoid organ development (80); lysosome (78); vasculature development (74); cell migration (68); regulation of protein polymerization (65); actin filament-based process (59); basolateral plasma membrane (46); fatty acid metabolism (42); peroxisome (34); SH2 domain (33); flavoprotein (32); regulation of MAPKKK cascade (30); GTP binding (26); valine, leucine and isoleucine degradation (23); histone deacetylase complex (16); T-helper 1 type immune response (8); phosphoprotein (1501); cytoplasm (699); acetylation (580); intracellular signaling cascade (244); endoplasmic reticulum (187); Golgi apparatus (184); cytosol (153); oxidoreductase (132); topological domain: lumenal (121); endosome (86); nucleolus (84); cell leading edge (44); cell soma (37); melanosome (35); pigment granule (35); colorectal cancer (34); gap junction (34); soluble fraction (33); lamellipodium (27); ruffle (21)
X	44–66	D	glycoprotein (226); kinase (37); cell junction (30); cell adhesion (25); immunoglobulin domain (21); neuromuscular junction (6); alternative splicing (170); plasma membrane (112); cell membrane (66)	DNA-binding (57)

^*Terms in plain type represent enriched clusters of functionally related genes identified using DAVID functional annotation [43], [44]. For each cluster with at least one annotation term with Benjamini FDR<0.10, the term with the lowest FDR is listed and the number of unique genes in the cluster is in parentheses. Significant annotation terms (FDR<0.10) not assigned to any cluster are listed in italics, and the number of unique genes in parentheses.