Table 1. Strains used in this study.
Strain | Genotype | |
KSC1635 | MATa-inc | MEC1-2HA::TRP1 sml1Δ::LEU2 ADH4cs::HIS2 |
KSC1645 | MATa-inc | mec1-KN-2HA::TRP1::URA3 sml1Δ::LEU2 ADH4cs::HIS2 |
KSC1333 | MATa | MEC1-2HA::TRP1 |
KSC3130 | MATa | MEC1-2HA::TRP1 ddc1Δ::LEU2 dpb11-1 |
KSC1636 | MATa-inc | MEC1-2HA::TRP1 ddc2Δ::LEU2 sml1Δ::LEU2 ADH4cs::HIS2 |
KSC2158 | MATa-inc | MEC1-2HA::TRP1 ddc2-S4::URA3 sml1Δ::LEU2 ADH4cs::HIS2 |
KSC2159 | MATa-inc | MEC1-2HA::TRP1 ddc2-KA::URA3 sml1Δ::LEU2 ADH4cs::HIS2 |
KSC1178 | MATa | sml1Δ::LEU2 |
KSC1234 | MATa | ddc2Δ::LEU2 sml1Δ::LEU2 |
KSC1340 | MATa | MEC1-2HA::TRP1 ddc2Δ::LEU2 sml1Δ::LEU2 |
KSC3153 | MATa | ddc2-S4::URA3 sml1Δ::LEU2 |
KSC3190 | MATa | ddc1Δ::LEU2 dpb11-1 sml1Δ::LEU2 |
KSC3244 | MATa | ddc2-S4::URA3 ddc1Δ::TRP1 dpb11-1 sml1Δ::LEU2 |
KSC3131 | MATa | ddc1Δ::LEU2 dpb11-1 |
KSC3308 | MATa | ddc2Δ::LEU2 [YCp-DDC2] |
PJ69-4A | MATa | GAL2-ADE2 GAL1-HIS3 |
KSC2077 | MATa | GAL2-ADE2 GAL1-HIS3 ddc2Δ::TRP1 sml1Δ::KanMX |
CBY51 | MATa | GAL-DDC1-GFP-LacI::URA3 RAD53-HA::LEU2 LacO256::TRP1 |
CBY88 | MATa | GAL-DDC1-GFP-LacI::URA3 GAL-DDC2-GFP-LacI::HIS3 RAD53-HA::LEU2 LacO256::TRP1 |
KSC2419 | MATa | GAL-DDC1-GFP-LacI::URA3 GAL-DDC2-S4-GFP-LacI::HIS3 RAD53-HA::LEU2 LacO256::TRP1 |
All the KSC strains, except for KSC2077 or KSC2419, are isogenic and derived from KSC006 (MATa ade1 his2 leu2 trp1 ura3) [7]. KSC2419 or KSC2077is derived from CBY51 or PJ69-4A, respectively. PJ69-4A has been described [7]. CBY51 and CBY88 have been obtained from David Toczyski [31]. MATa-inc is a mutation of the HO cleavage site [10].