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. 2014 Feb 20;8(2):e2661. doi: 10.1371/journal.pntd.0002661

Figure 4. Polarized entry of CHIKV at apical plasma membrane domain.

Figure 4

(A) The total virus yield at 24 h.p.i. of non-polarized Vero, polarized Vero C1008 and polarized HBMEC cells at an MOI of 10 was quantified by viral plaque assay. Entry of CHIKV is bi-directional in non-polarized Vero cells but occurs preferentially at the apical domain of polarized Vero C1008 and HBMEC cells. Two-tailed Student's t-test: * p<0.05, ** p<0.005. Vertical bars represent one standard deviation from the mean of three readings. (B) Virus binding assays show higher amount of CHIKV binding upon apical infection as compared to upon basolateral infection of polarized Vero C1008 cells. In contrast, the amount of CHIKV binding upon apical and basolateral infection of non-polarized Vero cells is similar. Two-tailed Student's t-test: *** p<0.001.