Figure 2.

On microscopic examination the vaginal mucosa was infiltrated by an adenocarcinoma of intermediate grade, with focal necrosis. Microphotographs of vaginal biopsy (A) showed the stroma infiltrated by neoplastic glands (yellow arrows) beneath the intact squamous epithelium of vagina [hematoxylin-and-eosin stain (H&E) magnification, 40×]. The immunostaining showed the neoplastic glands strongly positive for cytokeratin 20 (B) and CDX2 (C); the cytokeratin 7 and estrogen receptors were negative [400×]. On the basis of the morphology and immunohistochemical profile a diagnosis of vaginal infiltration by colonic adenocarcinoma was made.