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. 2014 Feb 20;9(2):e88887. doi: 10.1371/journal.pone.0088887

Figure 4. The zinc finger region of Nup358 is involved in nuclear import of hTERT-GFP.

Figure 4

A, Schematic representation of full-length Nup358 (amino acids (aa) 1–3223) and various Nup358-fragments used as rescue constructs. cc, predicted coiled-coil region, RB1-RB4, Ran-binding domains 1–4, IR, internal repeat, M, ‘middle domain’, CY, cyclophilin domain. vertical bars, FG-motifs; asterisk, region in corresponding RNA that confers resistance to siRNA. B, HeLa cells were treated with control siRNAs (not shown; compare upper left picture for localization of hTERT-GFP in cells with high levels of endogenous Nup358) or with siRNAs to deplete Nup358, and transfected with constructs coding for hTERT-GFP and HA-tagged fragments of Nup358, as indicated. Cells were fixed and analyzed by fluorescence microscopy. Antibodies against the C-terminal region of Nup358 were used to detect the endogenous protein, whereas the anti-HA antibody was used to detect Nup358-fragments. Bars, 10 or 20 µm. C, Quantification of the results shown in B. Error bars depict the standard deviation of the mean from three independent experiments.