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. 2014 Feb 20;9(2):e88887. doi: 10.1371/journal.pone.0088887

Figure 6. Nup358 and importin 7 cooperate in nuclear import of hTERT-GFP.

Figure 6

A, HeLa cells were treated with control siRNA or siRNAs against importin 7, Nup358 or both, and transfected with plasmids coding for wild-type hTERT-GFP (WT), the NLS-mutant (KR236AA = K236AR237A), myc-EZI or NES-GFP2-cNLS (-cNLS), as indicated. Cells were fixed, subjected to indirect immunofluorescence to detect myc-EZI or Nup358 (where appropriate) and analyzed by fluorescence microscopy. Bars, 10 µm. White arrow, cell with reduced level of endogenous Nup358 and with hTERT-GFP in the cytoplasm. B, Quantification of the results in A. Error bars depict the standard deviation of the mean from at least three independent experiments.