Figure 7. The engineered F-TrCP-Shc E3 ligase suppressed BT474 xenograft tumor growth in nude mice.

A. Nude mice were injected subcutaneously in the right flank with 5×10⁶ BT474 cells infected with pBMN-F-TrCP-Shc, pBMN-F-TrCP-Shc(m) or pBMN-GFP retroviruses. Tumor volumes were measured over a 4-week period and calculated by the formula: $(\text{length}\times \text{width}2)/2$. B. Tumor weight (g) was recorded at the end of the experiment. Columns, mean value of tumor weight; bars, SD. C. Representative images of tumor-bearing mice. * P<0.05 or **P<0.01 indicate tumors expressing pBMN-F-TrCP-Shc compared to those infected with pBMN-GFP or pBMN-F-TrCP-Shc(m).