Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 20;9(2):e89133. doi: 10.1371/journal.pone.0089133

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Burns et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Comparisons of periostin expression in wild-type E13.5 pc hearts (panels A, C) and Vcan ^(tm1Zim) E13.5 pc hearts (panels B,D) by confocal immunofluorescent localization. Periostin (green) expression in the Vcan ^(tm1Zim) hearts appeared more compact and less widely distributed throughout the cushions than in the wild-type. Boxes in panels A,B denote magnified areas shown in C,D. Periostin staining showed a sharper boundary between the apex of the interventricular septum (blue; α-sarcomeric myosin) and the adjacent cushion mesenchyme in the Vcan ^(tm1Zim) (D; arrow) as compared to that observed in wild-types (C; arrows). In adult hearts, periostin expression in the Vcan ^(tm1Zim) (F) appeared more intense in the aorta (Ao) walls and valve leaflets, however very little or no localization of periostin was observed in the interstitial tissue of the muscular interventricular septum (IVS) as compared to wild-type (E). In the Vcan ^(tm1Zim) heart, periostin appeared to localize only in zones adjacent to the myocardium (F;IVS), but did not show an interspersed pattern of expression between individual myocytes as seen in the wild-type IVS and aortic mitral continuity(E;IVS). Panels A & B same magnification bar = 150 µm; panels C & D same magnification bar = 50 µm; panels E & F same magnification bar = 200 µm.