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. 2014 Feb 20;9(2):e89133. doi: 10.1371/journal.pone.0089133

Figure 13. Western blot comparison of four proteins with altered abundance by itraq in Vcan (tm1Zim) mutant and wild-type hearts.

Figure 13

Proteins that showed different levels of altered abundance in the Vcan (tm1Zim) hearts are shown in the blot. Annexin A6 and Stathmin are proteins expressed in the heart that showed a relative decrease (0.85x and 0.73x respectively) in abundance in the Vcan (tm1Zim) mutant by iTRAQ and serphin1 (Hsp 47) that by iTRAQ showed increased abundance (1.55x). Additionally, Desmin that did not change with any significance between mutant (Mut) and wild-type (Wt) by iTRAQ, showed no significant change by western blot. Results of average relative density measurements of separately analyzed hearts are shown in the graph for each protein (for Stmn, Hsp47, Desmin n = 3; p<0.01; for Annexin A6 n = 2; p<0.0045).