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. 2014 Feb 20;9(2):e89117. doi: 10.1371/journal.pone.0089117

Figure 4. Camptothecin (CPT) modulates p53, androgen receptor and prostate specific antigen expression of LNCaP cells.

Figure 4

LNCaP cells were treated with different concentrations of CPT for 24 hours. Cells were lysed and androgen receptor (AR) and prostate specific antigen (PSA) expressions were determined by immunoblotting assays. (B) LNCaP cells were treated with 1 µM CPT or/and 1 nM R1881. Cells were lysed and expression of p53, AR, and PSA were determined by immunoblotting assay. (C) The pPSABH-transfected LNCaP cells (black bars) or the pPSABHE-transfected LNCaP cells (white bars) were treated with 0.5 µM CPT or/and 1 nM R1881 for 24 hours. Data are expressed as the mean percentage±SE of the stimulations of the PSA reporter activity. (D) Luciferase activity of MMTV reporter vector-transfected LNCaP cells after treatment with 0.5 µM CPT or/and 1 nM R1881 for 24 hours. Data are expressed as the mean percentage±SE of the stimulations of the MMTV reporter activity (*P<0.01).