Figure 1. Hybrid crosses and an example of AFLP gel showing bands of parental species and hybrids.

A) Interspecific cross and backcrosses used in experiments B) Selective PCR AFLP band patterns using primers with selective nucleotides GG (EcoRI) and CTG (MseI). The arrows indicate two instability markers detected in hybrids from backcross 1 as an example. MWM, molecular weight marker; Dk, D. koepferae; Db, D. buzzatii; HF1, F1 hybrids; BC1, backcross 1.