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| Element/Species | Chromosomes | |||||
| X | 2 | 3 | 4 | 5 | Total | |
| Osvaldo | ||||||
| D. buzzatii | G2, C | E4, D4 | D1, C | C | C | 4 |
| D. koepferae | C | C | C | C | C | 0 |
| Helena | ||||||
| D. buzzatii | A2 | E2 | G1, C | A5, D2 | 5 | |
| D. koepferae | A2, F3, G2 | B2, E2, F1 | D4, G2 | C4.1,C4.2,C5, F2 | 12 | |
| Galileo | ||||||
| D. buzzatii | E3 | 1 | ||||
| D. koepferae | G1 | 1 | ||||
C: centromere.
