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. 2014 Feb 20;9(2):e89121. doi: 10.1371/journal.pone.0089121

Figure 1. Bacterial induced TLR2/6 activation.

Figure 1

TLR2/6 expressing HEK transfectants were co-incubated with different strains of bifidobacteria or lactobacilli. (A) Dose response experiment indicating the effects of the different supplements (FBS = fetal bovine serum, HS = human serum, plasma = human plasma, MS = mouse serum) on TLR2/6 activity. TLR2/6 expressing cells were stimulated with B. breve NutRes 200 in a ratio of 15∶1 (bacteria:cell) for 16H. NFκB activity measured as Relative Light Units (RLU) was determined using a Luciferase reporting system as described in materials and methods. Relative NFκB activity was determined calculating the ratios between medium activity and the activity in the different serum fractions. (B) TLR2/6 activity induced by 3 Bifidobacteria and 3 Lactobacilli strains. TLR2/6-expressing cells were stimulated at a ratio of 15∶1 (bacteria:cell) in serum-free or 5% human serum (HS) containing medium. NFκB activity measured as RLU was determined as described above.