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. 2014 Feb 20;9(2):e89121. doi: 10.1371/journal.pone.0089121

Figure 2. EVs inhibit Bifidobacterium breve induced TLR2/6 activation.

Figure 2

TLR2/6-expressing HEK transfectants were stimulated with B. breve NutRes 200 in a ratio of 1∶15 for 16H. (A) Dose response experiment indicating the effects of the different human serum fractions on TLR2/6 activity (HS = intact human serum, HS-D = EV depleted human serum, HS-EV = human serum EVs in medium). NFκB activity was determined as described in Figure 1. (B) Data presented in Figure 2a were used to calculate Area Under the Curve (AUC) values. HS and HS-EVs dose-dependently inhibited TLR2/6 activity. Hash-signs indicate a significant difference (##P<0.01) compared to medium. EV depletion dose dependently rescued TLR2/6 activity. Asterisks indicate a significant difference (**P<0.01) compared to HS-D.