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. 2014 Feb 20;9(2):e89068. doi: 10.1371/journal.pone.0089068

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© 2014 Bondar et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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a. Confocal immunofluorescence for CXCR3 was performed in sections of duodenal biopsies. (i) Intraepithelial lymphocyte CXCR3⁺ cells in untreated CD patients are indicated by arrows. (ii). CXCR3⁺ cells were rarely observed in the intraepithelial compartment in non-CD controls. The epithelium is delimited by a thin line. CXCR3 is shown in green, and nuclei are shown in red. (Magnifications, 630× (i) and 1071× (ii)). b. Representative flow cytometric analysis from the epithelial compartment of a duodenal sample of an untreated CD patient showing IELs (CD3⁺ CD103⁺) that express CXCR3.