Table 2. Relative expression of the TubB gene in Phytophthora capsici isolates.
| Relative expression of PcTubB in the presence of zoxamide (µg ml−1)b | ||||||||
| Isolate | Sensitivitya | 0 | 0.1 | 0.5 | 1 | 5 | 10 | 50 |
| HX-1 | S | 1.0±0.0 | 2.2±0.2 | 2.6±0.5 | 2.3±0.2 | 2.8±0.5 | 1.9±0.2 | 2.3±0.4 |
| PCAS2 | S | 1.7±0.1 | 1.9±0.2 | 4.1±0.5 | 2.4±0.4 | 3.1±0.3 | 4.1±0.4 | 2.8±0.2 |
| PCAS1 | S | 1.0±0.0 | 1.1±0.1 | 1.0±0.1 | 1.1±0.2 | 1.1±0.1 | 0.7±0.1 | 1.6±0.1 |
| RZ3-5 | R | 1.2±0.1 | 2.5±0.3 | 2.4±0.0 | 2.2±0.3 | 1.3±0.0 | 1.8±0.0 | 1.4±0.0 |
| RZ13-2 | R | 1.5±0.1 | 1.6±0.1 | 2.1±0.1 | 2.0±0.3 | 1.9±0.1 | 1.6±0.2 | 2.4±0.2 |
| XH38-10 | R | 1.1±0.1 | 1.2±0.2 | 0.8±0.1 | 1.2±0.3 | 0.6±0.1 | 1.2±0.2 | 1.5±0.2 |
| XH38-13 | R | 1.0±0.1 | 3.1±0.2 | 3.7±0.6 | 3.2±0.2 | 3.5±0.5 | 2.2±0.8 | 3.1±0.3 |
a
Isolates were only considered resistant (R) when they were able to grow on PDA plates amended with 30 µg ml−1 zoxamide.
b
The expression of the PcTubB gene was normalized using the PcWS21 gene and then calibrated to the normalized PcTubB mRNA value of the HX-1 isolate in the absence of zoxamide. The mean and standard deviation values indicate the average relative expression between two independent biological experiments. The relative expression was determined in isolates subjected to zoxamide for 6 hours prior to mRNA isolation.