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. 2014 Feb 20;9(2):e89377. doi: 10.1371/journal.pone.0089377

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© 2014 Liu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–D) Double staining of Atoh1-HA and EGFP in control (A, C) and PLP/CreER^T+; Atoh1-HA+; α9AChR-EGFP+ (B, D) mice at P22 (A, B) and P90 (C, D). (E–H) Colabeling of Atoh1-HA and calbindin in control (E, G) and PLP/CreER^T+; Atoh1-HA+ (F, H) mice at P22 (E, F) and P90 (G, H). Arrows in (B), (D), (F), and (H) represent new IHCs. The inset in (B), (D), (F), and (H) shows a single-channel signal for Atoh1-HA. Arrow heads in (A–H) represent endogenous IHCs. OHCs: outer hair cells; IHC: inner hair cell. Scale bar: 20 µm.