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Histological section of heart tissue (200x) stained with toluidine blue to enhance the contour of the cardiomyocytes for measuring their circumference and immunohistochemistry using non-muscular β-actin to stain capillaries (brown). One cardiomyocyte cut in short axis and containing a nucleus (blue) is outlined and magnified (box) to show how cardiomyocyte circumference was measured (in arbitrary units).
