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. 2014 Feb 20;9(2):e89553. doi: 10.1371/journal.pone.0089553

Figure 5. TGFβ-induced expression of pro-metastatic proteins is upregulated in p53 null cells.

Figure 5

(a) OVCA 420 (p53 wild-type), OVCA 432 (p53 mutant), and SKOV3 (null p53) cells were treated with 10 ng/mL TGFβ for 24 hours and analyzed by western blotting. Membranes were probed with Maspin, TMEPAI and DKK1 primary antibodies. Actin was used as an internal loading control. (b) OVCA 420 cell lines were analyzed by western blot and probed for pro-metastatic factors TMEPAI, and DKK1. Actin was used as an internal loading control. (c) SKOV3 cell lines were analyzed by western blot and probed for pro-metastatic factors TMEPAI and DKK1. SKOV3 p53 WT was transiently transfected with 100 ng/mL of p53 wild-type plasmid. Actin was used as an internal loading control.