Table 1.

Description of the datasets used in this study and the statistical evaluation of age-associated probes

Tissue	Chronological datasets				Permuted datasets (N=100)
	Nr. samples	Age range	GT p-value *	Age-regulated **	Age-regulated mean ± SE**	[Ps>Cs/Ps<Cs]^
VL muscles	29	35-89	0.015	4101	2991 ± 86.1	7/93 [0.07]
Brain cortex	30	26-106	0.004	2339	677 ± 52.2	3/97 [0.03]
Kidney cortex	72	27-92	0.002	2397	1136 ± 77.2	11/89 [0.12]
Kidney medulla	61	29-92	0.205	1474	1090 ± 70.0	24/76 [0.31]

Table shows the four datasets used in this study in chronological order and permuted samples. The number of samples and the age range (in years) are indicated.

* The p-value determined with the globaltest (GT).

** The number of age-regulated probes (p-value < 0.05) was determined using a quadratic regression model and no age-association as null hypothesis. In the permuted datasets, the mean and the standard error (SE) from 100 permutations per dataset were calculated.

^The ratio between permuted samples (Ps) with a higher to lower number of significant probes, compared with chronologically ordered samples (Cs).