Fig. 4.

Glutamate caused a reduction in ATP concentration in cultured cortical neurons. AMSCs or CM prevented glutamate-induced ATP depletion. a A standard curve from an ATP assay showing a linear relationship between ATP levels and luminometer measurement values. b Total cellular ATP concentrations were evaluated with a luciferase-based ATP assay. Data are presented as mean±SD. ^*** P < 0.001 compared to control group, ^## P < 0.01, ^### P < 0.001 compared to glutamate treated group. One-Way ANOVA