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. 2014 Jan 22;13:13. doi: 10.1186/1475-2859-13-13

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Copyright © 2014 Yu et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Confirmation of the mutants that underwent the second homologous recombination. (A) Electrophoresis analysis of the PCR products to confirm the cross event. Lanes: M, DNA marker; 1 – 5, epsT deleted mutants; 6, wild type of SM9913. (B) Southern blot hybridization of the mutants and wild SM9913. Lanes: 1 – 4, the deleted mutants; 5, wild type of SM9913.