Table 3.

Primers used in this article

Primer	Sequence (5′-3′)	Description	Expected size (bp)
OriTF	GCCAGCTCGTCGGTGTAGC	OriT	700
OriTR	CAACAACGTTGCCCGGATCG
ZF	TGCACTGCAGCAAGACACTGTGAAGGC	RepA and its flanking sequences	2100
ZR	GATTCATTATAACCACGGATCCCTGCCTTTAAGATTTG
EmCFB	CAAATCTTAAAGGCAGGGATCCGTGGTTATAATGAATC	EmR in pOriT-4Em	825
EmC	GGACTAGTGTTAAGGGATGCAGTTTATG
UPF	ACGCGTCGACGTATGGTGCTGCTGATAACAGC	Upstream homologous fragment of epsT	834
UPR	CGCGGATCCCAGTGTGAGTAGCACCTCAC
DnF	CGGGATCCGTGAAAATGCGTACTAA	Downstream homologous fragment of epsT	830
DnR	CATTATAACCACTCTAGACCACTAAAGTTATCG
EmF	CGATAACTTTAGTGGTCTAGAGTGGTTATAATG	EmR in pMT	825
EmR	CTAGCTAGCGTTAAGGGATGCAGTTTATG
sacbF	CATGCCATGGCACATATACCTGCCGTTCAC	sacB	1900
sacBR	CCGGGCCCAATGCCAATAGGATATCGGC
TyzF	GACGATGAATGGAGTGGTAAGATAG	Confirmation of the second recombination	3400a
TyzR	CTCCAATCATGCTGCCATGTTGC		2000b

^athe size of the wild type.

^bthe size of the deleted mutant.