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Histamine stimulates Duox-mediated H2O2 production in HEK-293 cells stably expressing Duox1/DuoxA1α or Duox2/DuoxA2. HEK-293 cells stably expressing the Duox1/DuoxA1α (D1A1α) or the Duox2/DuoxA2 (D2A2) system were stimulated with 100 μM histamine, and H2O2 production was measured by the homovanillic acid/horseradish peroxidase assay. Representative kinetics and barographs with average ± SEM values are shown (n = 3).
