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. 2014 Feb;50(2):301–315. doi: 10.1165/rcmb.2012-0522OC

Figure 3.

Figure 3.

Effect of p38 MAPK inhibition on GR subcellular trafficking. (A) Top: Cells were treated with FP alone (100 nM) for 2 hours in the presence or absence of SB203580 (10 μM) added 1 hour before. (B) Top: Cells were transfected for 48 hours with 100 nM p38 siRNA or control siRNA before FP was added for an additional 2 hours. Cells were lysed, and cytosolic and nuclear extracts were prepared and assayed for GR and GAPDH by immunoblot analysis. Results are representative of three separate blots. Bottom: Scanning densitometry of three representative immunoblots with each condition normalized over the area density of the corresponding GAPDH content. The results are expressed as the fold increase over basal values. *P < 0.05 compared with untreated cells (A) or with untreated cells transfected with control siRNA (B). **P < 0.01 compared with untreated cells (A) or with untreated cells transfected with control siRNA (B). #P < 0.05 compared with untreated cells (A).