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. 2014 Feb;50(2):328–336. doi: 10.1165/rcmb.2013-0058OC

Figure 2.

Figure 2.

Vascular endothelial cadherin (VE-cadherin) translocates in response to simvastatin and mediates claudin-5 up-regulation. (A) ECs were either untreated or treated with simvastatin (5 μM, 4–48 h) and then subjected to membrane fractionation before Western blotting of cytosolic and membrane fractions for VE-cadherin (representative blots shown). (B) Immunofluorescence imaging of control ECs and simvastatin-treated ECs demonstrated membrane translocation of VE-cadherin in response to simvastatin at 16 hours. Scale bar, 10 μm. (C) Subsequently, ECs were transfected with nonspecific RNA (nsRNA) or silencing RNA (siRNA) specific for VE-cadherin (siVE-cad) before treatment with simvastatin (5 μM, 16 h), and then immunblotted for claudin-5 (representative blots shown). (D) Densitometry confirmed significantly reduced claudin-5 expression in simvastatin-treated ECs transfected with siVE-cad compared with simvastatin-treated EC controls (nsRNA) (*P < 0.05, n = 3/condition). Results are expressed as means (± SE).