Figure 4.

Effect of miRNA mimics on inflammatory agonist–induced PBEF/NAMPT transcription in human lung ECs. Total RNA was isolated from ECs, transfected with nc or with the indicated miRNA mimics (48 h) and untreated (A and B), exposed to 18% CS (A, 2 h), or treated with LPS (B, 4 h). PBEF/NAMPT mRNA levels were detected via real-time PCR. Transfection was performed with miR-1290 used as the second negative control. Data are presented as fold change in mRNA level over vehicle-treated control and expressed as means ± SE from four independent experiments.