Figure 5.

Effect of microRNA (miRNA) mimics on inflammatory agonist–induced PBEF/NAMPT expression in human lung ECs. ECs were transfected with nc or with the indicated miRNA mimics (48 h) and exposed to 18% CS (10 h) or LPS (B, 48 h), and endogenous PBEF/NAMPT protein was detected via immunoblots. The bar graphs represent relative densitometry (18% CS [A]; LPS [C]). Data are presented as fold changes in PBEF/NAMPT compared with nc or corresponding miRNA pretreatment without agonist challenge and expressed as means ± SE from three independent experiments. *P < 0.05 versus unstimulated control. ^#P < 0.01 versus unstimulated control.