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. 2014 Feb;50(2):409–418. doi: 10.1165/rcmb.2013-0292OC

Figure 6.

Figure 6.

Effect of miRNA mimics on inflammatory agonist–induced PBEF/NAMPT 3′-UTR reporter activity in human lung ECs. ECs were cotransfected with PBEF/NAMPT 3′-UTR reporter together with nc or with the indicated miRNA mimics and phRL-TK, a Renilla luciferase normalization control vector. ECs were untreated and exposed to 18% CS (A) or treated with LPS (C) (24 h) (B), and luciferase activity was measured according the manufacturer’s protocol. Data are presented as relative luciferase units over vehicle-treated control and expressed as means ± SE from four independent experiments. *Significantly different from control cells without stimulation (P < 0.05). **Significantly different from control cells stimulated with 18% CS or LPS (P < 0.05). #Significantly different from control cells stimulated with 18% CS or LPS (P < 0.01).