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Effect of the redox status of flavin on the dCRY-JET interaction. Wild-type or mutant dCRYs were mixed with GST-JET bound to glutathione-agarose beads and kept in the dark (D) or exposed to light (L) for 10 min. Then, the beads were collected by centrifugation and washed three times, and the bound proteins were detected by immunoblotting.
