FIGURE 7.
Inhibition of extracellular PSAP decreases membrane localization of GPR37 to GM1-dense lipid rafts. Representative images are shown for co-localization of GPR37 and CTxB in live cells at baseline (A) or after inhibition of extracellular PSAP (B). C, average Pearson's correlation of pixel intensity, as well as Manders' coefficient for fraction of green pixels overlapping red, was calculated. For each group, calculations were made from ROIs comprising the plasma membrane of each cell in 30 randomly selected fields of vision, nuntreated = 69 cells and nanti-PSAP = 70 cells. A marked reduction of the Pearson's correlation confirms reduced accumulation of GPR37 in GM1-dense membrane microdomains after inhibition of extracellular PSAP.