Comparison of 12.5% SDS-PAGE and Western blot profiles during purification of CfTX-A/B.
a, Coomassie-stained proteins; b, proteins bound to CfTX-1/2-specific antibodies; c, proteins bound to CfTX-A/B-specific antibodies. Lane 1, protein standards with molecular masses (kDa) indicated to the left; lane 2, crude C. fleckeri venom; lane 3, SEC Peak 2; lane 4, SEC Peak 3; lane 5, CEX Peak 2.